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JoVE Journal
Immunology and Infection

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Electroporation of Functional Bacterial Effectors into Mammalian Cells
 

Electroporation of Functional Bacterial Effectors into Mammalian Cells

Article doi: 10.3791/52296
January 19th, 2015

Summary January 19th, 2015

Electroporation was used to insert purified bacterial virulence effector proteins directly into living eukaryotic cells. Protein localization was monitored by confocal immunofluorescence microscopy. This method allows for studies on trafficking, function, and protein-protein interactions using active exogenous proteins, avoiding the need for heterologous expression in eukaryotic cells.

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