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Production of RNA for Transcriptomic Analysis from Mouse Spinal Cord Motor Neuron Cell Bodies by Laser Capture Microdissection
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Neuroscience
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JoVE Journal Neuroscience
Production of RNA for Transcriptomic Analysis from Mouse Spinal Cord Motor Neuron Cell Bodies by Laser Capture Microdissection

Production of RNA for Transcriptomic Analysis from Mouse Spinal Cord Motor Neuron Cell Bodies by Laser Capture Microdissection

DOI:
10.3791/51168-v

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11:46 min

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January 13, 2014

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, , ,
1Department of Genetics,Yale School of Medicine, 2Howard Hughes Medical Institute

Chapters

  • 00:05Title
  • 02:23Preparing Spinal Cord Sections
  • 04:21Staining Spinal Cord Sections
  • 07:20Laser Capture Microdissection (LMD)
  • 10:08Results: Motor Neuron Isolation
  • 11:07Conclusion

Summary

Automatic Translation

Automatic Translation

High-quality total RNA has been prepared from cell bodies of mouse spinal cord motor neurons by laser capture microdissection after staining spinal cord sections with Azure B in 70% ethanol. Sufficient RNA (~40-60 ng) is recovered from 3,000-4,000 motor neurons to allow downstream RNA analysis by RNA-seq and qRT-PCR.

Tags

Keywords: RNATranscriptomic AnalysisMouse Spinal CordMotor NeuronLaser Capture MicrodissectionAzure B StainingRNA ExtractionRNA-seqQRT-PCR

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