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Super-opløsning Imaging af neuronale Dense-core blærer
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Neuroscience
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JoVE Journal Neuroscience
Super-resolution Imaging of Neuronal Dense-core Vesicles

Super-opløsning Imaging af neuronale Dense-core blærer

DOI:
10.3791/51394-v

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09:30 min

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July 02, 2014

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, , ,
1Department of Physics,Lewis & Clark College, 2Program in Biochemistry and Molecular Biology,Lewis & Clark College, 3Jungers Center for Neuroscience Research,Oregon Health & Science University, 4Department of Chemistry,Lewis & Clark College

Chapters

  • 00:05Title
  • 01:12Image Acquisition
  • 05:36Image Processing, Display, and Analysis
  • 07:07Results: Representative PALM Images
  • 09:04Conclusion

Summary

Automatic Translation

Automatic Translation

Vi beskriver, hvordan man gennemfører fotoaktiveret lokalisering mikroskopi (PALM)-baserede studier af vesikler i faste, dyrkede neuroner. De centrale elementer i vores protokol omfatter mærkning blærer med photoconvertible kimærer, indsamling af spredte, indsamlede rå billeder med en super-opløsning mikroskopi system og behandling af RAW-billeder til at producere en super-opløsning.

Tags

Super-resolution ImagingNeuronal Dense-core VesiclesFluorescence MicroscopyPALMPhotoactivated Localization MicroscopyVesicle CargoHippocampal NeuronsDCVDense-core VesiclesExtrasynaptic Trafficking

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