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In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy
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In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy

In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy

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13,065 Views

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17:08 min

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August 06, 2014

DOI:

10.3791/51669-v

DOI:
10.3791/51669-v

•

17:08 min
August 06, 2014

•

2 Views
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1Light Microscopy Core Facility,NHLBI/NIH, 2Hematology Branch,NHLBI/NIH

Summary

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Combinatorial 5 fluorescent proteins marking of hematopoietic stem and progenitor cells allows in vivo clonal tracking via confocal and two-photon microscopy, providing insights into bone marrow hematopoietic architecture during regeneration. This method allows non-invasive fate mapping of spectrally-coded HSPCs-derived cells in intact tissues for extensive periods of time following transplantation.

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Malide, D., Métais, J., Dunbar, C. E. In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy. J. Vis. Exp. (90), e51669, doi:10.3791/51669 (2014).

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