Lentiviral Vector Transduction
Lentiviral Vector Transduction - Related Videos
03:07
Lentiviral Vector-Based Transduction of Rat Fertilized Embryos
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2026
Source: Koza, P., et al. Generation of Transgenic Rats using a Lentiviral Vector Approach. J. Vis. Exp. (2020)This video demonstrates microinjection-assisted delivery of lentiviral vectors into fertilized rat embryos to achieve stable transgene integration for the generation of transgenic rats.
10:58
A Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids
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Cited by 81 •
2015
In this video protocol we give a step by step explanation of lentiviral transduction in organoids of primary intestinal epithelium and of processing and downstream analysis of these cultures by quantitative RT-PCR, RNA-microarray and immunohistochemistry.
08:46
Production of Lentiviral Vectors for Transducing Cells from the Central Nervous System
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Cited by 44 •
2012
In this protocol we describe production, purification and titration of lentiviral vectors. We provide an example of lentiviral vector-mediated gene delivery in primary cultured neurons and astrocytes. Our methods may also apply to other cell types in vitro and in vivo.
02:30
Gene Delivery to Mouse Intestinal Organoids Using Lentiviral Vectors
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2026
Source: Van Lidth de Jeude, J. F., et al. Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids. J. Vis. Exp. (2015)This video describes the lentiviral transduction of mouse intestinal organoids from a crypt-like structure containing suspension. Using a basement membrane matrix as a scaffold and growth factor–supplemented media, lentiviral vectors are introduced to enable stable gene...
08:42
Dextran Enhances the Lentiviral Transduction Efficiency of Murine and Human Primary NK Cells
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Cited by 14 •
2018
The goal of this study was to formulate technologies that allow for successful gene transduction in primary natural killer (NK) cells. The dextran-mediated lentiviral transduction of human or mouse primary NK cells results in higher gene expression efficiencies. This method of gene transduction will vastly improve NK cell genetic manipulation.
FAQs
Reverse transcription converts the vector’s RNA genome into DNA, creating a form that can be integrated into the target-cell genome. Integration helps maintain expression of an introduced transgene or genetic regulator over time rather than limiting the experiment to a short-lived signal. This feature supports stable immune-cell engineering for functional studies of signaling, pathogen interactions, and disease mechanisms.
Because lentiviral vectors can introduce genetic material into both dividing and nondividing mammalian cells, researchers can study a broader range of cellular states. The method is therefore useful when immune-cell behavior depends on developmental status, activation, or limited proliferation. This flexibility also expands its relevance for investigating infection mechanisms and for developing vector-based therapeutic strategies.
A transgene can provide a defined cellular feature, such as a receptor or reporter, whereas a genetic regulator can alter the activity of an endogenous pathway. These choices let investigators either add a measurable function or perturb an existing response. In immunology, that distinction helps separate effects caused by receptor signaling from changes produced by modifying regulatory mechanisms.
The engineered vector must match the biological question and the target cell’s experimental role. A receptor can create a defined interaction system, a reporter can make pathway activity observable, and a genetic regulator can test causal contributions to cell behavior. Selecting among these cargo types connects the transduction strategy to specific questions about signaling, pathogen recognition, or immune function.
A typical study begins by engineering lentiviral particles to carry a selected transgene or genetic regulator, followed by introducing those particles to the mammalian target cells. The resulting cells can then be examined for the intended receptor, reporter, or perturbation in a relevant experimental system. This workflow links vector design with downstream analysis of cellular and infectious-disease mechanisms.
The approach is valuable when researchers need cells with defined genetic features to analyze pathogen interactions, signaling pathways, or immune-cell function. Engineered receptors can establish controlled interaction models, reporters can support analysis of response pathways, and gene perturbations can test mechanism. These applications make the technique relevant to both basic disease research and therapeutic vector development.