Human Ino80 Complex
Human Ino80 Complex - Related Videos
08:44
Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes
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Cited by 8 •
2014
This protocol describes a procedure for generating and purifying wild type and mutant versions of the human INO80 chromatin remodeling complex. Epitope tagged versions of INO80 subunits are stably expressed in HEK293 cells, and complete complexes and complexes lacking specific sets of subunits are purified by immunoaffinity chromatography.
14:03
Transduction of Human Cells with Polymer-complexed Ecotropic Lentivirus for Enhanced Biosafety
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Cited by 8 •
2011
Lentiviruses are a valuable research tool for exploring gene function; however, researchers may wish to avoid production of pantropic lentivirus encoding known or suspected oncogenes. As an alternative, we present a safer protocol for use of ecotropic lentivirus on human cells modified to express the ecotropic receptor mSlc7a1.
12:42
Probing for Mitochondrial Complex Activity in Human Embryonic Stem Cells
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Cited by 8 •
2008
In this video, we will show you how the mitochondrial respiratory chain complexes of human embryonic stem cells can be analyzed using in gel activity assays.
08:55
Characterization of Multi-subunit Protein Complexes of Human MxA Using Non-denaturing Polyacrylamide Gel-electrophoresis
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Cited by 3 •
2016
This article describes a simple and rapid protocol to evaluate the oligomeric state of the dynamin-like GTPase MxA protein from lysates of human cells using a combination of non-denaturing PAGE with western blot analysis.
08:42
Coordination Chemistry Complexes
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2023
Source: Laboratory of Dr. Neal Abrams — SUNY College of Environmental Science and Forestry Transition metals are found everywhere from vitamin supplements to electroplating baths. Transition metals also make up the pigments in many paints and compose all minerals. Typically, transition metals are found in the cationic form since they readily oxidize, or lose electrons, and are surrounded by electron donors called ligands. These ligands do not form ionic or covalent bonds with the metal center,...
FAQs
ATP hydrolysis provides the energy required by the catalytic INO80 subunit to reposition or otherwise alter nucleosomes. This energy-dependent activity can change how readily DNA is accessed within chromatin. The resulting structural changes allow the complex to participate in DNA-associated processes that require regulated access to the genome.
Associated subunits extend the complex beyond its catalytic activity by helping recognize chromatin and coordinate remodeling with DNA-associated processes. Their contribution links nucleosome changes to the cellular context in which remodeling is needed. This coordination is important because chromatin regulation must support genome maintenance as well as control of gene activity.
Nucleosome repositioning can regulate access to DNA that is embedded within chromatin. In the human INO80 complex, this capability connects chromatin organization with replication, transcription, and DNA-damage repair. It also becomes relevant when cells experience replication stress, because recovery requires coordinated management of chromatin and DNA-associated events.
The complex is examined in connection with DNA replication, transcription, DNA-damage repair, and recovery from replication stress. These areas represent distinct cellular demands for controlled DNA access and chromatin remodeling. Studying them together helps researchers determine how one remodeling assembly contributes both to genome maintenance and to regulation of gene-related activity.
Replication stress provides a context for studying how chromatin remodeling supports cellular recovery when DNA replication is challenged. Researchers examine INO80 in this setting to clarify how its ATP-dependent activity and associated subunits coordinate chromatin changes with DNA-associated processes. This work connects molecular remodeling mechanisms to preservation of genome stability.
Investigating defects in chromatin remodeling can show how disrupted control of DNA access affects genome maintenance and gene control. For INO80, this research may clarify why impaired complex function is relevant to disease biology. The broader outcome is a mechanistic understanding linking altered chromatin regulation with failures in genome stability.