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Imaging Intracellular Ca2+ Signals in Striatal Astrocytes from Adult Mice Using Genetically-encoded Calcium Indicators

Article DOI: 10.3791/51972-v • 12:19 min • November 19th, 2014
November 19th, 2014 •
1, 1, 2, 1,2
1Department of Physiology, University of California Los Angeles, 2Department of Neurobiology, University of California Los Angeles

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Summary
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The properties and functions of astrocyte intracellular Ca2+ signals in the striatum remain incompletely explored. We describe methods to express genetically encoded calcium indicators in striatal astrocytes using adeno-associated viruses of serotype 2/5 (AAV2/5), as well as procedures to reliably image Ca2+ signals within striatal astrocytes in situ.

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Intracellular Ca2+ Signals Striatal Astrocytes Genetically-encoded Calcium Indicators GECIs Astrocyte [Ca2+]i Signals Microcircuits Striatum Surgical Methods Confocal Imaging GECI Expression Astrocyte Reactivity Brain Slice Imaging Conditions
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