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Imaging Intracellular Ca2+ Signals in Striatal Astrocytes from Adult Mice Using Genetically-encoded Calcium Indicators
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JoVE Journal Neuroscience
Imaging Intracellular Ca2+ Signals in Striatal Astrocytes from Adult Mice Using Genetically-encoded Calcium Indicators

Imaging Intracellular Ca2+ Signals in Striatal Astrocytes from Adult Mice Using Genetically-encoded Calcium Indicators

DOI:
10.3791/51972-v

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12:19 min

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November 19, 2014

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, , ,
1Department of Physiology,University of California Los Angeles, 2Department of Neurobiology,University of California Los Angeles

Chapters

  • 00:05Title
  • 01:35Prepare Micropipette and AAV2/5 Virus Loading
  • 03:13Craniotomy and Microinjections
  • 05:53Acute Brain Slice Preparation for Confocal Ca2+ Imaging
  • 07:19Confocal [Ca2+]i Imaging
  • 10:09Results: GECIs and Ca2+ Signaling in Astrocytes
  • 11:42Conclusion

Summary

Automatic Translation

Automatic Translation

The properties and functions of astrocyte intracellular Ca2+ signals in the striatum remain incompletely explored. We describe methods to express genetically encoded calcium indicators in striatal astrocytes using adeno-associated viruses of serotype 2/5 (AAV2/5), as well as procedures to reliably image Ca2+ signals within striatal astrocytes in situ.

Tags

Keywords: Intracellular CalciumAstrocytesStriatumGenetically-encoded Calcium IndicatorsGCaMPIn VivoIn SituConfocal ImagingMicrocircuitsCalcium Signaling

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