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JoVE Journal
Neuroscience

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Regioselective Biolistic Targeting in Organotypic Brain Slices Using a Modified Gene Gun
 

Regioselective Biolistic Targeting in Organotypic Brain Slices Using a Modified Gene Gun

Article doi: 10.3791/52148
October 24th, 2014

Summary October 24th, 2014

Recent improvements in organotypic brain slice preparations have permitted their exploitation for biotechnological applications. Organotypic slices maintain local structural characteristics of in vivo biology, including functional synaptic connections. Here we present a regioselective biolistic delivery method to label and genetically manipulate these slices.

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