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06:40 min
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October 24, 2014
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Summary
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Recent improvements in organotypic brain slice preparations have permitted their exploitation for biotechnological applications. Organotypic slices maintain local structural characteristics of in vivo biology, including functional synaptic connections. Here we present a regioselective biolistic delivery method to label and genetically manipulate these slices.
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Cite this Article
Arsenault, J., Nagy, A., Henderson, J. T., O'Brien, J. A. Regioselective Biolistic Targeting in Organotypic Brain Slices Using a Modified Gene Gun. J. Vis. Exp. (92), e52148, doi:10.3791/52148 (2014).
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