Dna Coated Gold Particles
Dna Coated Gold Particles - Related Videos
10:38
Synthesis of Functionalized 10-nm Polymer-coated Gold Particles for Endothelium Targeting and Drug Delivery
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Cited by 8 •
2018
We describe a method of synthesizing biocompatible 10-nm gold nanoparticles, functionalized by coating poly-ethylene glycol onto the surface. These particles can be used in vitro and in vivo for delivering therapeutics to nanoscale cellular and extracellular spaces that are difficult to access with conventional nanoparticle sizes.
08:42
Determination of Zeta Potential via Nanoparticle Translocation Velocities through a Tunable Nanopore: Using DNA-modified Particles as an Example
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Cited by 7 •
2016
Here we use a polyurethane tunable nanopore integrated into a resistive pulse sensing technique to characterize nanoparticles surface chemistry via the measurement of particle translocation velocities, which can be used to determine the zeta potential of individual nanoparticles.
05:02
Growth of Gold Dendritic Nanoforests on Titanium Nitride-coated Silicon Substrates
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Cited by 5 •
2019
This study presents a feasible procedure for synthesizing gold dendritic nanoforests on titanium nitride/silicon substrates. The thickness of gold dendritic nanoforests increases linearly within 15 min of a synthesis reaction.
03:37
Subatomic Particles
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2020
Dalton was only partially correct about the particles that make up matter. All matter is composed of atoms, and atoms are composed of three smaller subatomic particles: protons, neutrons, and electrons. These three particles account for the mass and the charge of an atom. The Discovery of the Electron The first clue about the subatomic structure came at the end of the 19th century when J.J. Thomson discovered the electron using a cathode ray tube. This apparatus consisted of a sealed glass...
08:55
Hydroquinone Based Synthesis of Gold Nanorods
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Cited by 11 •
2016
This paper describes a protocol for the synthesis of gold nanorods, based on the use of hydroquinone as reducing agent, plus the different mechanisms for controlling their size and aspect ratio.
FAQs
The delivery sequence depends on two linked events: DNA first becomes associated with the gold surface through adsorption or precipitation, and a burst of gas then supplies the force needed to propel the coated particles into tissue. Once particles reach cells, the DNA is released sufficiently to support gene expression, connecting physical transport with the biological readout.
A physical particle-based approach is valuable when tissue is difficult to transfect because it does not depend on introducing genetic material through a biological carrier. In neuroscience, this provides an alternative to viral methods and supports experiments that require delivery to a selected region. The resulting localization can help relate gene expression to nearby neuronal structure or circuit organization.
Because particles can be directed into selected regions, investigators can examine localized gene function rather than treating an entire tissue as uniformly exposed. This spatial restriction is especially useful in brain slices or living tissue, where neighboring neuronal populations may differ in connectivity or cellular responses. It therefore links the delivery site with interpretation of circuit-level observations.
A basic workflow begins by preparing DNA on the gold particle surface, either by adsorption or precipitation. The coated particles are then accelerated with a burst of gas into the selected tissue. After cellular entry, investigators assess DNA-driven expression in the exposed region. This sequence separates particle preparation, physical delivery, and biological expression, making each stage relevant to experimental planning and interpretation.
In neuroscience, the approach can support fluorescent labeling of neurons, morphological reconstruction, and localized gene-function studies. Fluorescent labels help identify transfected cells, while morphology-focused analyses use their visible structure to examine neuronal form. Because delivery can be confined to selected regions, the same strategy also helps investigate neuronal connectivity and cellular responses within brain slices or living tissue.
Using DNA-coated gold particles in brain slices or living tissue allows investigators to study neural questions in distinct experimental contexts while retaining localized delivery. In either setting, expression can reveal cellular responses or support visualization of neuronal structure. This flexibility extends the method from controlled slice-based analysis to studies of connectivity and gene function in living neural tissue.