Assessment of Dendritic Arborization in the Dentate Gyrus of the Hippocampal Region in Mice

Devsmita Das; Cristy Phillips; Bill Lin; Fatemeh Mojabi; Mehmet Akif Baktir; Van Dang; Ravikumar Ponnusamy; Ahmad Salehi

doi:10.3791/52371

Journal

/

Neuroscience

/

Assessment of Dendritic Arborization in the Dentate Gyrus of the Hippocampal Region in Mice

JoVE Journal
Neuroscience

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Neuroscience

Assessment of Dendritic Arborization in the Dentate Gyrus of the Hippocampal Region in Mice

10,216 Views

•

10:55 min

•

March 31, 2015

DOI:

10.3791/52371-v

DOI:

10.3791/52371-v

•

10:55 min

March 31, 2015

•

2 Views

Devsmita Das^1,2, Cristy Phillips³, Bill Lin¹, Fatemeh Mojabi¹, Mehmet Akif Baktir², Van Dang^1,2, Ravikumar Ponnusamy¹, Ahmad Salehi^1,2

¹VA Palo Alto Health Care System, ²Department of Psychiatry and Behavioral Sciences,Stanford University School of Medicine, ³Department of Physical Therapy,Arkansas State University

Summary

We describe two methods for visualization and quantification of dendritic arborization in the hippocampus of mouse models: real-time and extended depth of field imaging. While the former method allows sophisticated topographical tracing and quantification of the extent of branching, the latter allows speedy visualization of the dendritic tree.