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Assessment of Dendritic Arborization in the Dentate Gyrus of the Hippocampal Region in Mice
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JoVE Journal Neuroscience
Assessment of Dendritic Arborization in the Dentate Gyrus of the Hippocampal Region in Mice

Assessment of Dendritic Arborization in the Dentate Gyrus of the Hippocampal Region in Mice

DOI:
10.3791/52371-v

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10:55 min

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March 31, 2015

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, , , , , , ,
1VA Palo Alto Health Care System, 2Department of Psychiatry and Behavioral Sciences,Stanford University School of Medicine, 3Department of Physical Therapy,Arkansas State University

Chapters

  • 00:05Title
  • 01:39Sectioning and Embedment
  • 03:30Visualization of Brain Sections
  • 06:43Extended Depth of Field Imaging
  • 08:07Generating Extremely High Resolution Images
  • 09:07Results: Visualization of Dendritic Arborization with and without EDFI Methods
  • 09:58Conclusion

Summary

Automatic Translation

Automatic Translation

We describe two methods for visualization and quantification of dendritic arborization in the hippocampus of mouse models: real-time and extended depth of field imaging. While the former method allows sophisticated topographical tracing and quantification of the extent of branching, the latter allows speedy visualization of the dendritic tree.

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Keywords: Dendritic ArborizationHippocampusDentate GyrusNeuronal StructureNeuroplasticityImaging TechniquesStaining MethodsQuantificationWild-type Mice

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