Assessment of Dendritic Arborization in the Dentate Gyrus of the Hippocampal Region in Mice

Devsmita Das; Cristy Phillips; Bill Lin; Fatemeh Mojabi; Mehmet Akif Baktir; Van Dang; Ravikumar Ponnusamy; Ahmad Salehi

doi:10.3791/52371

Assessment of Dendritic Arborization in the Dentate Gyrus of the Hippocampal Region in Mice

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Neuroscience

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JoVE Journal Neuroscience

Assessment of Dendritic Arborization in the Dentate Gyrus of the Hippocampal Region in Mice

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10:55 min

March 31, 2015

DOI: 10.3791/52371-v

Devsmita Das^1,2, Cristy Phillips³, Bill Lin¹, Fatemeh Mojabi¹, Mehmet Akif Baktir², Van Dang^1,2, Ravikumar Ponnusamy¹, Ahmad Salehi^1,2

¹VA Palo Alto Health Care System, ²Department of Psychiatry and Behavioral Sciences,Stanford University School of Medicine, ³Department of Physical Therapy,Arkansas State University

We describe two methods for visualization and quantification of dendritic arborization in the hippocampus of mouse models: real-time and extended depth of field imaging. While the former method allows sophisticated topographical tracing and quantification of the extent of branching, the latter allows speedy visualization of the dendritic tree.

The overall goal of the following experiment is to study the extent of dendritic arborization in the hippocampus of a mouse model. This is achieved through two different methods. In the first method, dendrites are traced manually in real time throughout the entire thickness of each section.

Following tracing, the entire dendritic tree can be reconstructed and analyzed. Using the fan diagram analysis of various fan diagrams derived from different mice can be used as an objective means of comparison. The second method is to visualize dendritic arborization with extended depth of field imaging.

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