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A Multi-detection Assay for Malaria Transmitting Mosquitoes
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A Multi-detection Assay for Malaria Transmitting Mosquitoes

Article DOI: 10.3791/52385-v • 09:00 min • February 28th, 2015
February 28th, 2015 •
1, 1, 1, 2, 1
1Department of Pathology, Microbiology and Immunology, School of Veterinary Medicine, University of California - Davis, 2Veterinary Genetics Laboratory, School of Veterinary Medicine, University of California, Davis

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Summary
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Malaria transmitting mosquitoes have a number of epidemiologically important characteristics that can only be detected using molecular techniques. Utilizing a MALDI-TOF based SNP genotyping platform, we developed an assay for simultaneously detecting multiple key traits (species, insecticide resistance, parasite infection and host choice) of malaria vectors.

Tags

Multi-detection Assay Malaria Transmitting Mosquitoes Anopheles Gambiae Species Complex Molecular Assays Epidemiological Studies Species Identification Insecticide Resistance Parasite Infection Status Host Preference Polymerase Chain Reaction (PCR) KDR Mutations DDT And Pyrethroid Insecticides Enzyme-linked Immunosorbent Assays (ELISAs) Plasmodium Parasite DNA Detection PCR Assays Host-specific DNA Multi-detection Assay (MDA) Multiplex Reaction Genotyping 33SNPs False Results
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