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Protocol for Production of a Genetic Cross of the Rodent Malaria Parasites
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Chapters
- 00:05Title
- 01:16Introduction
- 01:37Infection of Laboratory Mice with Blood-Stage Malaria Parasites
- 04:06Mixed Clone Infection of Mice
- 05:14Feeding Mosquitoes
- 06:45Dissecting Mosquito Midget and Counting Oocysts
- 08:58Collecting Sporozoites from Mosquitoes by Centrifugation
- 10:56Cloning Progeny from a Genetic Cross Using Limiting Dilution
- 12:57Production of Recombinant Malaria Parasites by Genetic Cross
- 13:11Conclusion
Genetic crosses of rodent malaria parasites are performed by feeding two genetically distinct parasites to mosquitoes. Recombinant progeny are cloned from mouse blood after allowing mosquitoes to bite infected mice. This video shows how to produce genetic crosses of Plasmodium yoelii and is applicable to other rodent malaria parasites.
Tags
Genetic CrossRodent Malaria ParasitesAntimalarial DrugsLinkage MappingGenesLociPlasmodium YoeliiLaboratory AdaptationBiologic CharacteristicsGenetic MarkersMicrosatellite MarkersAFLP MarkersPlasmodium FalciparumTechniquesDrs. David WallikerRichard CarterUniversity Of EdinburghMice Mus MusculusGametocytesPhenotypesMosquitoes
