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Evaluating the Effectiveness of Cancer Drug Sensitization In Vitro and In Vivo
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JoVE Journal Medicine
Evaluating the Effectiveness of Cancer Drug Sensitization In Vitro and In Vivo

Evaluating the Effectiveness of Cancer Drug Sensitization In Vitro and In Vivo

DOI:
10.3791/52388-v

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09:19 min

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February 06, 2015

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, , , , ,
1Microbiology and Immunology,Western University, 2Biochemistry,Western University, 3Surgery,Western University, 4Oncology,Western University

Chapters

  • 00:05Title
  • 01:30Biosensor Chip Preparation and Cell Seeding
  • 03:05Metabolism Measurement
  • 04:47CAM Experiment Drug Treatment and Injection
  • 07:11Results: Cancer Drug Sensitization
  • 08:52Conclusion

Summary

Automatic Translation

Automatic Translation

Here, real-time monitoring of tumor cell metabolism, combined with an in vivo chicken embryo chorio-allantoic membrane (CAM) model of metastasis, are used to evaluate novel anti-cancer targets/agents for their ability to sensitize tumor cells to DNA damaging chemotherapeutics.

Tags

Keywords: Cancer Drug SensitizationDNA Repair InhibitorsBRCA2 InhibitionCisplatinTumor Cell MetabolismReal-time MonitoringChicken Embryo Chorioallantoic Membrane (CAM) ModelExtravasationInvasionCancer Therapy Evaluation

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