Forward Genetics Screens Using Macrophages to Identify <em>Toxoplasma gondii </em>Genes Important for Resistance to IFN-&#947;-Dependent Cell Autonomous Immunity

Odaelys Walwyn; Sini Skariah; Brian Lynch; Nathaniel Kim; Yukari Ueda; Neal Vohora; Josh Choe; Dana G. Mordue

doi:10.3791/52556

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Forward Genetics Screens Using Macrophages to Identify Toxoplasma gondii Genes Important for Resistance to IFN-γ-Dependent Cell Autonomous Immunity

Forward Genetics Screens Using Macrophages to Identify <em>Toxoplasma gondii </em>Genes Important for Resistance to IFN-γ-Dependent Cell Autonomous Immunity

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Forward Genetics Screens Using Macrophages to Identify Toxoplasma gondii Genes Important for Resistance to IFN-γ-Dependent Cell Autonomous Immunity

Forward Genetics Screens Using Macrophages to Identify Toxoplasma gondii Genes Important for Resistance to IFN-γ-Dependent Cell Autonomous Immunity

11,039 Views

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11:21 min

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March 12, 2015

DOI:

10.3791/52556-v

DOI:

10.3791/52556-v

•

11:21 min

March 12, 2015

•

11030 Views

Odaelys Walwyn*¹, Sini Skariah*¹, Brian Lynch¹, Nathaniel Kim¹, Yukari Ueda¹, Neal Vohora¹, Josh Choe¹, Dana G. Mordue¹

¹Department of Microbiology and Immunology,New York Medical College

Summary

Forward genetics is a powerful approach to identify genes in intracellular pathogens important for resistance to cell autonomous immunity. The current approach uses innate immune cells, specifically macrophages, to identify novel Toxoplasma gondii genes important for immune evasion.