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Preparation of Single-cell Suspensions for Cytofluorimetric Analysis from Different Mouse Skin Regions
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Immunology and Infection
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JoVE Journal Immunology and Infection
Preparation of Single-cell Suspensions for Cytofluorimetric Analysis from Different Mouse Skin Regions

Preparation of Single-cell Suspensions for Cytofluorimetric Analysis from Different Mouse Skin Regions

DOI:
10.3791/52589-v

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05:58 min

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April 20, 2016

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, , ,
1Department of Biotechnology and Biosciences,University of Milano-Bicocca, 2Boston Children’s Hospital, Division of Gastroenterology,Harvard Medical School, 3Humanitas Clinical and Research Center

Chapters

  • 00:05Title
  • 00:44Skin Sample Collection
  • 02:29Skin Sample Immunostaining
  • 04:11Flow Cytometric Analysis
  • 04:59Results: Quantitative Analysis of Mouse Immune Cells from Different Regions of the Skin
  • 05:32Conclusion

Summary

Automatic Translation

Automatic Translation

The skin is home to a complex immune cell network. We describe an efficient methodology for the digestion of mouse skin, from different parts of the animal's body, in order to obtain a single-cell suspension and analyze the different leukocyte populations resident in the skin by flow cytometry.

Tags

Keywords: Single-cell SuspensionCytofluorimetric AnalysisMouse SkinResident Immune CellsSkin SamplingSkin DigestionSkin Cell Labeling

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