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JoVE Journal
Immunology and Infection

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Preparation of Single-cell Suspensions for Cytofluorimetric Analysis from Different Mouse Skin Regions
 

Preparation of Single-cell Suspensions for Cytofluorimetric Analysis from Different Mouse Skin Regions

Article DOI: 10.3791/52589
April 20th, 2016

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Summary April 20th, 2016

The skin is home to a complex immune cell network. We describe an efficient methodology for the digestion of mouse skin, from different parts of the animal's body, in order to obtain a single-cell suspension and analyze the different leukocyte populations resident in the skin by flow cytometry.

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