Journal
/
Medicine
/
Utilizing Murine Inducible Telomerase Alleles in the Studies of Tissue Degeneration/Regeneration and Cancer
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal Medicine
Utilizing Murine Inducible Telomerase Alleles in the Studies of Tissue Degeneration/Regeneration and Cancer

Utilizing Murine Inducible Telomerase Alleles in the Studies of Tissue Degeneration/Regeneration and Cancer

DOI:
10.3791/52599-v

•

08:34 min

•

April 13, 2015

•

, , , , , ,
1Department of Cancer Biology,UT MD Anderson Cancer Center, 2Novartis Institutes for Biomedical Research, 3Sanofi US, 4Institute of Applied Cancer Science,UT MD Anderson Cancer Center

Chapters

  • 00:05Title
  • 01:35Reactivation of Telomerase in mTERT-ER and LSL-mTERT Mice In Vivo
  • 02:33Isolation of Neural Stem Cells and Reactivation of Telomerase In Vitro
  • 05:07Telomere FISH
  • 06:37Results: Effect of Telomerase Reactivation
  • 08:01Conclusion

Summary

Automatic Translation

Automatic Translation

Telomere and telomerase play essential roles in ageing and tumorigenesis. The goal of this protocol is to show how to generate two murine inducible telomerase knock-in alleles and how to utilize them in the studies of tissue degeneration/regeneration and cancer.

Tags

Keywords: Telomere DysfunctionTelomerase ReactivationCancerNeural Stem CellsMurine Models4-Hydroxytamoxifen (4-OHT)Lox-Stopper-LoxTERT (LSL-mTERT)Telomere FISHMetaphase Chromosomes

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/52599?article_type=v

A Method for Murine Islet Isolation and Subcapsular Kidney Transplantation
check_url/52599?article_type=v

An Orthotopic Model of Murine Bladder Cancer
check_url/52599?article_type=v

Utilizing Transcranial Magnetic Stimulation to Study the Human Neuromuscular System
check_url/52599?article_type=v

Immunohistochemical Staining of B7-H1 (PD-L1) on Paraffin-embedded Slides of Pancreatic Adenocarcinoma Tissue
check_url/52599?article_type=v

A Preclinical Murine Model of Hepatic Metastases
check_url/52599?article_type=v

An In Vitro Dormancy Model of Estrogen-sensitive Breast Cancer in the Bone Marrow: A Tool for Molecular Mechanism Studies and Hypothesis Generation
check_url/52599?article_type=v

The bm12 Inducible Model of Systemic Lupus Erythematosus (SLE) in C57BL/6 Mice
check_url/52599?article_type=v

Utilizing an Orally Dissolving Strip for Pharmacological and Toxicological Studies: A Simple and Humane Alternative to Oral Gavage for Animals
check_url/52599?article_type=v

Pre-clinical Orthotopic Murine Model of Human Prostate Cancer
check_url/52599?article_type=v

Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis

Read Article