Journal
/
Medicine
/
Utilization of the Soft Agar Colony Formation Assay to Identify Inhibitors of Tumorigenicity in Breast Cancer Cells
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Utilization of the Soft Agar Colony Formation Assay to Identify Inhibitors of Tumorigenicity in Breast Cancer Cells

Utilization of the Soft Agar Colony Formation Assay to Identify Inhibitors of Tumorigenicity in Breast Cancer Cells

DOI:
10.3791/52727-v

•

11:06 min

•

May 20, 2015

•

, , , ,
1Department of Baker Institute for Animal Health,Cornell University, 2Department of Molecular Biology and Genetics,Cornell University, 3Department of Biochemistry and Molecular Pharmacology,University of Massachusetts Medical School

Chapters

  • 00:05Title
  • 02:02Preparation of the Bottom Layer: 0.6% Agarose Gel
  • 04:39Preparation of the Cell-containing Layer: 0.3% Agarose Gel
  • 06:44Preparation of the Feeder Layer: 0.3% Agarose Gel
  • 08:08Data Collection
  • 09:10Results: Colony Formation in BB-CLA-treated MCF10DCIS Cells
  • 10:40Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we document the use of the soft agar colony formation assay to test the effects of a peptidylarginine deiminase (PADI) enzyme inhibitor, BB-Cl-amidine, on breast cancer tumorigenicity in vitro.

Tags

Keywords: Soft Agar Colony Formation AssayTumor Progression3D Cellular EnvironmentCell ProliferationCell MigrationCell TransformationGenetic PerturbationsPADI2Breast CancerBB-Cl-amidineTumorigenicityMCF10DCIS Cells

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/52727?article_type=v

A Strategy to Identify de Novo Mutations in Common Disorders such as Autism and Schizophrenia
check_url/52727?article_type=v

Multi-modal Imaging of Angiogenesis in a Nude Rat Model of Breast Cancer Bone Metastasis Using Magnetic Resonance Imaging, Volumetric Computed Tomography and Ultrasound
check_url/52727?article_type=v

Live Imaging of Drug Responses in the Tumor Microenvironment in Mouse Models of Breast Cancer
check_url/52727?article_type=v

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells
check_url/52727?article_type=v

Three Dimensional Cultures: A Tool To Study Normal Acinar Architecture vs. Malignant Transformation Of Breast Cells
check_url/52727?article_type=v

Orthotopic Injection of Breast Cancer Cells into the Mammary Fat Pad of Mice to Study Tumor Growth.
check_url/52727?article_type=v

An Ex vivo Model to Study Hormone Action in the Human Breast
check_url/52727?article_type=v

Mammosphere Formation Assay from Human Breast Cancer Tissues and Cell Lines
check_url/52727?article_type=v

In Vivo and Ex Vivo Approaches to Study Ovarian Cancer Metastatic Colonization of Milky Spot Structures in Peritoneal Adipose
check_url/52727?article_type=v

Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies

Read Article