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Alginate Encapsulation of Pluripotent Stem Cells Using a Co-axial Nozzle
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JoVE Journal Developmental Biology
Alginate Encapsulation of Pluripotent Stem Cells Using a Co-axial Nozzle

Alginate Encapsulation of Pluripotent Stem Cells Using a Co-axial Nozzle

DOI:
10.3791/52835-v

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07:13 min

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July 02, 2015

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,
1Center for International Research on Integrative Biomedical System, Institute of Industrial Science,The University of Tokyo

Chapters

  • 00:05Title
  • 01:32Cell Encapsulation into Alginate Hydrogel Capsules and Treatment of Alginate Capsules
  • 03:44Culture and Collect Cells in Alginate Capsules
  • 04:56Results: Visualizations of Alginate Capsule Formation and Morphologies of Mouse iPS Cells in Capsules
  • 06:43Conclusion

Summary

Automatic Translation

Automatic Translation

We established a method of encapsulating pluripotent stem cells (PS cells) into alginate hydrogel capsules using a co-axial nozzle. This prevents cells from aggregating excessively and limits the shear stress experienced by cells in suspension culture. The technique is applicable to the mass production of PS cells as well as research on stem cell niche.

Tags

Alginate EncapsulationPluripotent Stem CellsCo-axial NozzleSuspension CultureAggregationShear StressCapsule SizeAlg-Ca CapsuleAlg-PLL CoatingStem Cell NicheMass Production

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