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A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

DOI:
10.3791/53051-v

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11:32 min

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November 23, 2015

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, , , , , , ,
1Materials Engineering Division,Lawrence Livermore National Laboratory, 2Department of Biomedical Engineering,Boston University

Chapters

  • 00:05Title
  • 01:04Cleanroom Fabrication of Microfluidic Chips for Acoustophoresis
  • 02:32Piezo Transducer Attachement
  • 03:51Separation Procedure
  • 08:26Automated Cleaning and Decontamination
  • 09:08Results: Size Separation of Biological Particles Using an Acoustic Microdevice
  • 10:24Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes a system architecture for performing automated small volume (0.15–1.5 ml) particle separations using a microfluidic device, and discusses methods to optimize acoustofluidic device performance and operation.

Tags

Keywords: MicrofluidicsSample ProcessingAcoustic MicrodeviceParticle SeparationAutomationIntegrationSize SeparationBiological ParticlesReal-time FeedbackSample Conservation

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