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Microfluidic Acoustophoresis for Flowthrough Separation of Gram-Negative Bacteria using Aptamer Affinity Beads

Article DOI: 10.3791/63300-v • 06:08 min • October 17th, 2022
October 17th, 2022 •
1, 2, 3, 1,2,4
1Department of Digital Anti-Aging Health Care, Inje University, 2Micro device Lab Co., Ltd, 3Biohealth Products Research Center (BPRC), Inje University, 4Department of Biomedical Engineering, Inje University

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This paper describesthe fabrication and operation of microfluidic acoustophoretic chips using the microfluidic acoustophoresis technique and aptamer-modified microbeads that can be used for fast, efficient isolation of Gram-negative bacteria from a medium.

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Microfluidic Acoustophoresis Flowthrough Separation Gram-negative Bacteria Aptamer Affinity Beads Microfluidic Acoustophoresis Chip PS Beads PS Sample Mixture Borosilicate Glass PZT Cyanoacrylate Adhesive Luria-Bertani Medium Incubate Centrifuge PBS Buffer Binding Buffer Streptavidin Coated Micro Bead Mixture
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