Journal
/
Engineering
/
Microfluidic Acoustophoresis for Flowthrough Separation of Gram-Negative Bacteria using Aptamer Affinity Beads
JoVE Journal
Engineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Engineering
Microfluidic Acoustophoresis for Flowthrough Separation of Gram-Negative Bacteria using Aptamer Affinity Beads

Microfluidic Acoustophoresis for Flowthrough Separation of Gram-Negative Bacteria using Aptamer Affinity Beads

DOI:
10.3791/63300-v

•

06:08 min

•

October 17, 2022

•

, , ,
1Department of Digital Anti-Aging Health Care,Inje University, 2Micro device Lab Co., Ltd, 3Biohealth Products Research Center (BPRC),Inje University, 4Department of Biomedical Engineering,Inje University

Chapters

  • 00:04Introduction
  • 00:45Microuidic Acoustophoresis Chip Design and Fabrication
  • 01:23Bacterial Strains and Culture
  • 01:56Microbeads and Immobilization of Aptamer onto Microbeads
  • 03:10Acoustophoresis Setup and Operation
  • 04:19Results: Using Microfluidics to Capture GN Bacteria from Culture Samples at High Speed
  • 05:26Conclusion

Summary

Automatic Translation

Automatic Translation

This paper describesthe fabrication and operation of microfluidic acoustophoretic chips using the microfluidic acoustophoresis technique and aptamer-modified microbeads that can be used for fast, efficient isolation of Gram-negative bacteria from a medium.

Tags

Keywords: Microfluidic AcoustophoresisGram-negative Bacteria SeparationAptamer Affinity BeadsFlowthrough SeparationCell IsolationMicrofluidic DevicePZTBiotinylated DNA AptamerStreptavidin-coated MicrobeadsLuria-Bertani MediumCentrifugationTris-HCl BufferBSA

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/63300?article_type=v

Terahertz Microfluidic Sensing Using a Parallel-plate Waveguide Sensor
check_url/63300?article_type=v

Picoinjection of Microfluidic Drops Without Metal Electrodes
check_url/63300?article_type=v

Spatial Separation of Molecular Conformers and Clusters
check_url/63300?article_type=v

Fabrication and Testing of Microfluidic Optomechanical Oscillators
check_url/63300?article_type=v

Phase Diagram Characterization Using Magnetic Beads as Liquid Carriers
check_url/63300?article_type=v

Capillary-based Centrifugal Microfluidic Device for Size-controllable Formation of Monodisperse Microdroplets
check_url/63300?article_type=v

Solvent Bonding for Fabrication of PMMA and COP Microfluidic Devices
check_url/63300?article_type=v

A Modular Microfluidic Technology for Systematic Studies of Colloidal Semiconductor Nanocrystals
check_url/63300?article_type=v

A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms
check_url/63300?article_type=v

One-Step Approach to Fabricating Polydimethylsiloxane Microfluidic Channels of Different Geometric Sections by Sequential Wet Etching Processes

Read Article