Journal
/
Bioengineering
/
Assembly and Operation of an Acoustofluidic Device for Enhanced Delivery of Molecular Compounds to Cells
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Assembly and Operation of an Acoustofluidic Device for Enhanced Delivery of Molecular Compounds to Cells

Assembly and Operation of an Acoustofluidic Device for Enhanced Delivery of Molecular Compounds to Cells

DOI:
10.3791/62035-v

•

07:16 min

•

January 21, 2021

•

, , , , , , , ,
1Department of Bioengineering,University of Louisville, 2School of Medicine,University of Louisville, 3Department of Biology,University of Louisville

Chapters

  • 00:04Introduction
  • 00:45Fabrication of Acoustofluidic Device
  • 02:18Assembly and Operation of Acoustofluidic System
  • 04:08Preparation of Ultrasound Contrast Agents
  • 05:38Results: Acoustofluidic Treatment Enhances Intracellular Molecular Delivery
  • 06:30Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the assembly and operation of a low-cost acoustofluidic device for rapid molecular delivery to cells via sonoporation induced by ultrasound contrast agents.

Tags

Keywords: Acoustofluidic DeviceBiomolecule DeliveryCell TransformationPDMSPhotoresist WaferPiezo TransducerMicrocontroller8 MHz SignalTygon Tubing

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/62035?article_type=v

In vitro Assembly of Semi-artificial Molecular Machine and its Use for Detection of DNA Damage
check_url/62035?article_type=v

Operation of a Benchtop Bioreactor
check_url/62035?article_type=v

A Novel Three-dimensional Flow Chamber Device to Study Chemokine-directed Extravasation of Cells Circulating under Physiological Flow Conditions
check_url/62035?article_type=v

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery
check_url/62035?article_type=v

Ordering Single Cells and Single Embryos in 3D Confinement: A New Device for High Content Screening
check_url/62035?article_type=v

Synthesis of Cationized Magnetoferritin for Ultra-fast Magnetization of Cells
check_url/62035?article_type=v

Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen
check_url/62035?article_type=v

Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
check_url/62035?article_type=v

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions
check_url/62035?article_type=v

Fibroblast Derived Human Engineered Connective Tissue for Screening Applications

Read Article