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A New Approach that Eliminates Handling for Studying Aggression and the “Loser” Effect in Drosophila melanogaster
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
A New Approach that Eliminates Handling for Studying Aggression and the “Loser” Effect in Drosophila melanogaster
DOI:

07:19 min

December 30, 2015

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Chapters

  • 00:05Title
  • 01:22Social Isolation of Pupae – Day 1
  • 02:11Fly Painting for Identification – Day 5
  • 02:47Preparation of the Apparatus and Individual Fighting Chambers
  • 03:43Behavioral Experiments
  • 05:24Results: Observed Loser Effects
  • 06:07Conclusion

Summary

Automatic Translation

During fruit fly fights, the behavioral patterns observed, fight dynamics, and associated learning and memory are influenced by experimental conditions. The protocol presented here describes a novel procedure that entirely eliminates handling of flies during experiments. This improves fight dynamics and allows formation of strong "loser" effects.

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