During fruit fly fights, the behavioral patterns observed, fight dynamics, and associated learning and memory are influenced by experimental conditions. The protocol presented here describes a novel procedure that entirely eliminates handling of flies during experiments. This improves fight dynamics and allows formation of strong "loser" effects.
Trannoy, S., Chowdhury, B., Kravitz, E. A. A New Approach that Eliminates Handling for Studying Aggression and the "Loser" Effect in Drosophila melanogaster. J. Vis. Exp. (106), e53395, doi:10.3791/53395 (2015).