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Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery Method
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery Method

Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery Method

DOI:
10.3791/53825-v

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10:53 min

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February 26, 2018

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, , , , , , ,
1Cell Types Program,Allen Institute for Brain Science, 2The Ben and Catherine Ivy Center for Advanced Brain Tumor Treatment,Swedish Neuroscience Institute

Chapters

  • 00:04Title
  • 01:05Setup of the Slicing Station
  • 03:15Brain Dissection and Slicing
  • 06:32Optimized NMDG Protective Recovery Procedure
  • 08:05Results: Improved Neuronal Preservation and Reliability of Gigaohm Seal Formation with the Optimized NMDG Protective Recovery Method
  • 09:38Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol demonstrates the implementation of an optimized N-methyl-D-glucamine (NMDG) protective recovery method of brain slice preparation. A single media formulation is used to reliably obtain healthy brain slices from animals of any age and for diverse experimental applications.

Tags

Keywords: Brain SlicesNMDG Protective Recovery MethodPatch Clamp ElectrophysiologyNeuronal PreservationAdult Mouse BrainNMDG HEPES ACSFBrain Slice Recovery ChamberHEPES ACSFAgarose Embedding

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