Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / Preparation of Acute Brain Slices Using an…
JoVE Journal
Neuroscience

This content is Open Access.

JoVE Journal Neuroscience
Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery Method
Organotypic Slice Cultures to Study Oligodendrocyte Dynamics and Myelination
Journal (Neuroscience)
Organotypic Slice Cultures to Study Oligodendrocyte Dynamics and Myelination
Determination of Photoreceptor Cell Spectral Sensitivity in an Insect Model from In Vivo Intracellular Recordings
Journal (Neuroscience)
Determination of Photoreceptor Cell Spectral Sensitivity in an Insect Model from In Vivo Intracellular Recordings
 

Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery Method

Article DOI: 10.3791/53825-v • 10:53 min • February 26th, 2018
February 26th, 2018 •
*1, *1, 1, 1, 2, 1, 1, 1
1Cell Types Program, Allen Institute for Brain Science, 2The Ben and Catherine Ivy Center for Advanced Brain Tumor Treatment, Swedish Neuroscience Institute
* These authors contributed equally

Chapters

Summary
Automatic Translation

This protocol demonstrates the implementation of an optimized N-methyl-D-glucamine (NMDG) protective recovery method of brain slice preparation. A single media formulation is used to reliably obtain healthy brain slices from animals of any age and for diverse experimental applications.

Tags

Brain Slices N-Methyl-D-glucamine Optimized Method Patch Clamp Electrophysiology Synaptic Properties Neuronal Preservation Patch Clamp Recording Adult Mouse Brain Tissue Slicing Station Tissue Slicer Machine Surgical Instruments Zirconium Ceramic Injector Blade Specimen Holder NMDG HEPES ACSF Brain Slice Recovery Chamber
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter