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Differentiation of Atrial Cardiomyocytes from Pluripotent Stem Cells Using the BMP Antagonist Grem2
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Developmental Biology
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JoVE Journal Developmental Biology
Differentiation of Atrial Cardiomyocytes from Pluripotent Stem Cells Using the BMP Antagonist Grem2

Differentiation of Atrial Cardiomyocytes from Pluripotent Stem Cells Using the BMP Antagonist Grem2

DOI:
10.3791/53919-v

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08:52 min

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March 10, 2016

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1Department of Medicine, Division of Cardiovascular Medicine,Vanderbilt University School of Medicine, 2Department of Pharmacology,Vanderbilt University, 3Department of Cell & Developmental Biology,Vanderbilt University School of Medicine

Chapters

  • 00:05Title
  • 00:33Murine Embryonic Stem Cell (mESC) Culture and Passaging
  • 03:16Embryoid Body (EB) Preparation and Grem2 Treatment
  • 06:12Results: Grem2-induction of Cardiomyocyte Differentiation
  • 08:00Conclusion

Summary

Automatic Translation

Automatic Translation

Generating cardiomyocytes from pluripotent stem cells in vitro allows access to large amounts of cardiac tissue in vitro for basic science and clinical applications. This protocol uses the atrializing factor Grem2 to both increase the numbers of cardiomyocytes obtained and to generate cardiomyocytes with an atrial phenotype.

Tags

Keywords: Atrial CardiomyocytesPluripotent Stem CellsBMP Antagonist Grem2Cardiac DevelopmentCardiac DiseasesAtrial ArrhythmiaMouse Embryonic Stem Cells (mESCs)Cell CultureCell DifferentiationTrypsin EDTACell PassagingCell Confluence

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