Sarcomere Shortening of Pluripotent Stem Cell-Derived Cardiomyocytes using Fluorescent-Tagged Sarcomere Proteins.

It is difficult to assess sarcomere function in the pluripotent stem cell-derived cardiomyocytes due to their underdeveloped and disorganized sarcomere. Using fluorescent-tagged sarcomere proteins, we can now access sarcomere shortening. We can visualize sarcomeres and assess their function in vitro using stem cell-derived cardiomyocytes and fluorescent-tagged sarcomere proteins.

Using AAV-based transduction, we can expand the method to any patient-derived induced pluripotent stem cells. This method can be expanded to the development of cardiomyopathy therapy, as it can be used to directly assess disease-related sarcomere dysfunction in vitro. This method is useful in the cardiology field, including pediatric cardiology study.