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JoVE Journal
Immunology and Infection

This content is Open Access.

Antibody Binding Specificity for Kappa (Vκ) Light Chain-containing Human (IgM) Antibodies
 

Antibody Binding Specificity for Kappa (Vκ) Light Chain-containing Human (IgM) Antibodies: Polysialic Acid (PSA) Attached to NCAM as a Case Study

Article doi: 10.3791/54139
June 29th, 2016

Summary June 29th, 2016

We present a protocol to identify protein moieties containing antigens for human and mouse IgM antibodies with specific kappa (Vκ) light chains. This protocol is not limited to IgM class antibodies but applies to all immunoglobulin isotypes that target their antigen with sufficiently high affinity during immunoprecipitations.

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