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Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells
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Immunology and Infection
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JoVE Journal Immunology and Infection
Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells

DOI:
10.3791/54263-v

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15:29 min

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August 05, 2016

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, , , , , ,
1Focal Area Infection Biology, Biozentrum,University of Basel, 2Centre d’Immunologie de Marseille-Luminy,Université de la Méditérannée UM2, INSERM U1104 CNRS UM7280, 3Departmento de Microbiologìa and Instituto de Salud Tropical,Universidad de Navarra, 4BioDataAnalysis GmbH

Chapters

  • 00:05Title
  • 00:54Reverse siRNA Transfection
  • 03:19Infection and Fixation
  • 05:55Staining and Imaging
  • 08:14Automated Image Analysis
  • 12:21Results: Automated High-throughput Quantification of Brucella Infection in HeLa Cells
  • 14:06Conclusion

Summary

Automatic Translation

Automatic Translation

Two assays for microscopy-based high-throughput screening of host factors involved in Brucella infection are described. The entry assay detects host factors required for Brucella entry and the endpoint assay those required for intracellular replication. While applicable for alternative approaches, siRNA screening in HeLa cells is used to illustrate the protocols.

Tags

Keywords: MicroscopyHigh-throughput ScreeningBrucella InfectionHeLa CellsHost FactorsSiRNACell CultureTransfectionAutomated WorkflowImage Analysis

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