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JoVE Journal
Immunology and Infection

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Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells
 

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells

Article DOI: 10.3791/54263 15:29 min August 5th, 2016
August 5th, 2016

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Summary

Two assays for microscopy-based high-throughput screening of host factors involved in Brucella infection are described. The entry assay detects host factors required for Brucella entry and the endpoint assay those required for intracellular replication. While applicable for alternative approaches, siRNA screening in HeLa cells is used to illustrate the protocols.

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Microscopy-based Assays High-throughput Screening Host Factors Brucella Infection HeLa Cells Host-pathogen Interaction Semi-automated Workflow Image Analysis Robots Efficient High-throughputs SiRNA Screens Screening Plates Transfection Medium Transfection Reagent Complex Formation HeLa Cell Culture
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