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JoVE Journal
Developmental Biology

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Rapid Acquisition of 3D Images Using High-resolution Episcopic Microscopy
 

Rapid Acquisition of 3D Images Using High-resolution Episcopic Microscopy

Article doi: 10.3791/54625
November 21st, 2016

Summary November 21st, 2016

We describe a detailed protocol using high-resolution episcopic microscopy to acquire three-dimensional (3D) images of mouse embryos. This improved protocol utilizes a modified tissue preparation method to enhance penetration of the fluorescent dye, thereby permitting morphometric analysis of both small and large-sized specimens.

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