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可诱导T7 RNA聚合酶介导的多基因表达系统,pMGX
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Genetics
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JoVE Journal
Genetics
Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX
Please note that all translations are automatically generated.
Click here for the English version.
可诱导T7 RNA聚合酶介导的多基因表达系统,pMGX
DOI:
10.3791/55187-v
•
10:09 min
•
June 27, 2017
•
Mohamed I. Hassan*
1
,
Fern R. McSorley*
1
,
Kinya Hotta
,
Christopher N. Boddy
1
Department of Chemistry and Biomolecular Sciences
,
University of Ottawa
,
2
School of Biosciences
,
The University of Nottingham Malaysia Campus
Chapters
00:05
Title
00:49
Cloning Genes of Interest in the Multigene Expression System Vector, pMGX
04:11
Insert Gene 2 into the pMGX Vector Containing Gene 1, pMGX-
yfg1
07:08
Results: Multigene Expression in pMGX
09:10
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该研究描述了使用pMGX质粒系统在
大肠杆菌中
使用单个质粒的多个基因的T7介导的共表达的方法。
Tags
Inducible T7 RNA Polymerase
Multigene Expression System
PMGX
Synthetic Biology
Biochemistry
Protein Complexes
Co-expression
Escherichia Coli
Gene Cloning
Restriction Enzyme Digestion
Gel Electrophoresis
Gel Extraction
Ligation
Bacterial Transformation
Antibiotic Selection
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