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Modeling Amyloid-β42 Toxicity and Neurodegeneration in Adult Zebrafish Brain
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Neuroscience
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JoVE Journal Neuroscience
Modeling Amyloid-β42 Toxicity and Neurodegeneration in Adult Zebrafish Brain

Modeling Amyloid-β42 Toxicity and Neurodegeneration in Adult Zebrafish Brain

DOI:
10.3791/56014-v

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10:01 min

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October 25, 2017

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, , , , , ,
1German Centre for Neurodegenerative Diseases (DZNE) Dresden within Helmholtz Association, 2B CUBE, Center for Molecular Bioengineering,Technische Universität Dresden, 3Center for Regenerative Therapies Dresden (CRTD),TU Dresden

Chapters

  • 00:05Title
  • 01:04Preparation of Aβ42 Peptide
  • 04:40Preparation of Injection Apparatus
  • 06:09Tissue Preparation and Sectioning
  • 07:23Immunohistochemical Staining and Microscopy
  • 08:31Results: Purification and Immunohistochemistry of Amyloid-β42 in Adult Zebrafish Brain
  • 09:24Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the synthesis, characterization, and injection of monomeric amyloid-β42 peptides for generating amyloid toxicity in adult zebrafish to establish an Alzheimer's disease model, followed by histological analyses and detection of aggregations.

Tags

Keywords: Amyloid-β42Alzheimer’s DiseaseAdult Zebrafish BrainNeural Stem CellsNeurodegenerationPeptide SynthesisFMOCHBTUDMFNMMCleavageHPLC

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