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JoVE Journal
Immunology and Infection

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Generation of Escape Variants of Neutralizing Influenza Virus Monoclonal Antibodies
 

Generation of Escape Variants of Neutralizing Influenza Virus Monoclonal Antibodies

Article doi: 10.3791/56067
August 29th, 2017

Summary August 29th, 2017

We describe a method by which we identify critical residues required for the binding of human or murine monoclonal antibodies that target the viral hemagglutinin of influenza A viruses. The protocol can be adapted to other virus surface glycoproteins and their corresponding neutralizing antibodies.

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