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Journal / Biology / 慢性肝损伤小鼠胆碱缺乏、氨基补充的饮食模型…
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慢性肝损伤小鼠胆碱缺乏、氨基补充的饮食模型
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慢性肝损伤小鼠胆碱缺乏、氨基补充的饮食模型

Article DOI: 10.3791/56138-v • 07:28 min • October 21st, 2017
October 21st, 2017 •
1, 1, 2, 3, 1,4, 1, 5,6,7, 8,9, 10,11, 1,12
1School of Biomedical Sciences & Curtin Health Innovation Research Institute, Curtin University, 2School of Public Health & Curtin Health Innovation Research Institute, Curtin University, 3Institute of Biochemistry, Christian-Albrechts-University, 4School of Veterinary and Life Sciences, Murdoch University, 5Centenary Institute of Cancer Medicine and Cell Biology, The University of Sydney, 6Royal Prince Alfred Hospital, 7A.W. Morrow Gastroenterology and Liver Centre, 8QIMR Berghofer Medical Research Institute, 9Faculty of Medicine and Biomedical Sciences, The University of Queensland, 10Fiona Stanley and Fremantle Hospitals, 11School of Medical and Health Sciences, Edith Cowan University, 12School of Medicine and Pharmacology, University of Western Australia

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在这里, 我们描述了一个常见的方法来诱导慢性肝损伤小鼠通过喂养的胆碱缺乏和氨基补充 (多) 饮食。我们展示健康监测, 肝脏灌注, 隔离和保存。六周的时间过程可以告知肝损伤、病理、纤维化、炎症和肝祖细胞反应。

Tags

细胞生物学 问题 128 小鼠模型 胆碱缺乏 氨基补充饮食 慢性肝病 肝脏灌注 肝脏隔离 肝祖细胞 炎症反应 组织学 纤维化 肝脏微环境
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