Journal
/
Neuroscience
/
Whole-cell Currents Induced by Puff Application of GABA in Brain Slices
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Whole-cell Currents Induced by Puff Application of GABA in Brain Slices

Whole-cell Currents Induced by Puff Application of GABA in Brain Slices

DOI:
10.3791/56387-v

•

07:32 min

•

October 12, 2017

•

, , ,
1School of Psychology,South China Normal University, 2School of Life Sciences,South China Normal University, 3Brain Science Institute,South China Normal University, 4Center for Studies of Psychological Application,South China Normal University, 5Guangdong Key Laboratory of Mental Health and Cognitive Science,South China Normal University

Chapters

  • 00:05Title
  • 00:54Preparation of Prefrontal Cortex Slice
  • 02:57Whole-cell Patch Recording
  • 05:57Results: Dose and Puff-duration Response Curves
  • 06:30Conclusion

Summary

Automatic Translation

Automatic Translation

We describe the puff technique, by which pharmacological reagents can be administered during whole-cell patch-clamp recording, and highlight various aspects of the features that are crucial for its success.

Tags

Keywords: Whole-cell CurrentsGABABrain SlicesPuff ApplicationElectrophysiologyPharmacological AdministrationAcute Prefrontal Cortex SliceVibratomeACSFRecording ElectrodesPuff MicropipettesSodium Channel Blocker

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/56387?article_type=v

GABA-activated Single-channel and Tonic Currents in Rat Brain Slices
check_url/56387?article_type=v

Local Application of Drugs to Study Nicotinic Acetylcholine Receptor Function in Mouse Brain Slices
check_url/56387?article_type=v

Fast Micro-iontophoresis of Glutamate and GABA: A Useful Tool to Investigate Synaptic Integration
check_url/56387?article_type=v

Paired Whole Cell Recordings in Organotypic Hippocampal Slices
check_url/56387?article_type=v

Whole-cell Patch-clamp Recordings in Brain Slices
check_url/56387?article_type=v

Application of Automated Image-guided Patch Clamp for the Study of Neurons in Brain Slices
check_url/56387?article_type=v

Evaluation of Synapse Density in Hippocampal Rodent Brain Slices
check_url/56387?article_type=v

Recording and Modulation of Epileptiform Activity in Rodent Brain Slices Coupled to Microelectrode Arrays
check_url/56387?article_type=v

Preparation of Acute Spinal Cord Slices for Whole-cell Patch-clamp Recording in Substantia Gelatinosa Neurons
check_url/56387?article_type=v

Assessment of Long-term Depression Induction in Adult Cerebellar Slices

Read Article