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Neuroscience

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Assay Development for High Content Quantification of Sod1 Mutant Protein Aggregate Formation in Living Cells
 

Assay Development for High Content Quantification of Sod1 Mutant Protein Aggregate Formation in Living Cells

Article doi: 10.3791/56425
October 4th, 2017

Summary October 4th, 2017

We describe a method to quantify the aggregation of misfolded proteins. Our protocol details lentiviral induced stable cell line generation, automated confocal imaging, and image analysis of protein aggregates. As an illustrative application, we studied the effect of small molecules in promoting SOD1 aggregation in a time- and dose-dependent manner.

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