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Journal / Biochemistry / Deep Proteome Profiling by Isobaric Labeling,…
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Biochemistry

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JoVE Journal Biochemistry
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Article DOI: 10.3791/56474-v • 10:37 min • November 15th, 2017
November 15th, 2017 •
1, 1, 1, 2,3, 1, 1, 2, 1,2
1St. Jude Proteomics Facility, St. Jude Children's Research Hospital, 2Departments of Structural Biology and Developmental Neurobiology, St. Jude Children's Research Hospital, 3Heilongjiang University of Chinese Medicine

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Summary
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We present a protocol to accurately quantitate proteins with isobaric labelling, extensive fractionation, bioinformatics tools, and quality control steps in combination with liquid chromatography interfaced to a high-resolution mass spectrometer.

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Deep Proteome Profiling Isobaric Labeling Liquid Chromatography Mass Spectrometry Software-assisted Quantification Proteins Cell Lysate Drug Treatments Time Courses Biological Conditions Medicine Biology Biomarker Discovery Human Diseases Cell Lysis Fluid Estimation Downstream Digestion Labeling Separation Procedures Cultured Cells PBS Wash Centrifuge Supernatant Tissue Collection And Storage
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