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An In Vivo Duo-color Method for Imaging Vascular Dynamics Following Contusive Spinal Cord Injury
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
An In Vivo Duo-color Method for Imaging Vascular Dynamics Following Contusive Spinal Cord Injury
DOI:

09:25 min

December 31, 2017

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Chapters

  • 00:05Title
  • 01:22External Jugular Vein Catheterization
  • 03:09Spine Stabilization, C5 – C7 Laminectomy, and Two-photon (2P) Imaging Window Installation
  • 05:02First Fluorescent Dye Injection and Baseline Imaging
  • 06:25Louisville System Apparatus (LISA) Device-induced C7 Contusive Injury, Second Fluorescent Dye Injection, and Post-injury Imaging
  • 07:26Results: Representative In Vivo Vascular Dynamic Imaging Following Contusive Spinal Cord Injury
  • 08:27Conclusion

Summary

Automatic Translation

We introduce an in vivo imaging method using two different fluorescent dyes to track dynamic spinal vascular changes following a contusive spinal cord injury in adult Sprague-Dawley rats.

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