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Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA
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Collection and Extraction of Occupational Air Samples for Analysis of Fungal DNA

Article DOI: 10.3791/56730-v • 12:02 min • May 2nd, 2018
May 2nd, 2018 •
1, 1, 1
1Allergy and Clinical Immunology Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Centers for Disease Control and Prevention

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Summary
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Determining the fungal diversity within an environment is a method utilized in occupational health studies to identify health hazards. This protocol describes DNA extraction from occupational air samples for amplification and sequencing of fungal ITS regions. This approach detects many fungal species that can be overlooked by traditional assessment methods.

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Occupational Air Sample Fungal DNA Extraction Sequencing-based Approach Fungal Hazards Negative Health Effects Air-sampling Filter Cassette Cellulose Filter Support Pad Extension Cowl NIOSH Aerosol Sampler Sealing Tape Collection Tubes
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