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JoVE Journal
Immunology and Infection

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Size Matters: Measurement of Capsule Diameter in Cryptococcus neoformans

Article DOI: 10.3791/57171 08:24 min February 27th, 2018
February 27th, 2018



The polysaccharide capsule is the primary virulence factor in Cryptococcus neoformans, and its size correlates with strain virulence. Capsule diameter measurements are used in phenotypic testing and to gauge therapeutic efficacy. Here a standard method of capsule induction is presented, and two methods of staining and measuring diameter are compared.



Capsule Diameter C. Neoformans Staining Technique Imaging Technique Measurement Virulence Automation Capsule Production Incubation Yeast Peptone Dextrose Broth Log Phase Centrifugation Wash Resuspension DMEM Concentration Carbon Dioxide India Ink
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