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JoVE Journal
Immunology and Infection

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Size Matters: Measurement of Capsule Diameter in Cryptococcus neoformans

Article DOI: 10.3791/57171
February 27th, 2018


Summary February 27th, 2018

The polysaccharide capsule is the primary virulence factor in Cryptococcus neoformans, and its size correlates with strain virulence. Capsule diameter measurements are used in phenotypic testing and to gauge therapeutic efficacy. Here a standard method of capsule induction is presented, and two methods of staining and measuring diameter are compared.


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