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JoVE Journal
Biochemistry

This content is Open Access.

Variations on Negative Stain Electron Microscopy Methods
 

Variations on Negative Stain Electron Microscopy Methods: Tools for Tackling Challenging Systems

Article doi: 10.3791/57199
February 6th, 2018

Summary February 6th, 2018

Negative stain EM is a powerful technique for visualizing macromolecular structure, but different staining techniques can produce varying results in a sample dependent manner. Here several negative staining approaches are described in detail to provide an initial workflow for tackling the visualization of challenging systems.

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