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Variations on Negative Stain Electron Microscopy Methods

Variations on Negative Stain Electron Microscopy Methods: Tools for Tackling Challenging Systems

Article DOI: 10.3791/57199 06:06 min February 6th, 2018
February 6th, 2018



Negative stain EM is a powerful technique for visualizing macromolecular structure, but different staining techniques can produce varying results in a sample dependent manner. Here several negative staining approaches are described in detail to provide an initial workflow for tackling the visualization of challenging systems.



Negative Stain Electron Microscopy Variations Techniques Preparation EM Samples Grid Preparation Carbon Sheet Method Negative Staining Reagents Glow Discharge Unit Hydrophilic Side Blot Method Sample Adsorption Capillary Action Filter Paper Ultrapure Water Volatile Buffer Solution
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