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Journal / Biochemistry / Single Particle Cryo-Electron Microscopy: From…
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Biochemistry

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JoVE Journal Biochemistry
Single Particle Cryo-Electron Microscopy
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Single Particle Cryo-Electron Microscopy: From Sample to Structure

Article DOI: 10.3791/62415-v • 11:52 min • May 29th, 2021
May 29th, 2021 •
1, 1, 2, 2, 2, 2, 1, 1
1Astbury Centre Structural Molecular Biology, School Molecular and Cellular Biology, Faulty Biological Sciences, University of Leeds, 2Diamond Light Source, Harwell Science and Innovation Campus

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Summary
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Structure determination of macromolecular complexes using cryoEM has become routine for certain classes of proteins and complexes. Here, this pipeline is summarized (sample preparation, screening, data acquisition and processing) and readers are directed towards further detailed resources and variables that may be altered in the case of more challenging specimens.

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Single-particle Cryo-EM Sample Preparation Structure Determination Grid Screening Data Collection Cryo-EM Facilities Remote Grid Screening Single-particle Data Acquisition Auto-loader Tab Microscope User Interface Inventory Button Cassette Cartridge Presence Grid Transfer Microscope Column EPU Software Acquisition Optics And Settings Atlas Preset Beam Setting Presets
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