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Chapters
- 00:04Title
- 01:01Intravenous Injection of Evans Blue Dye
- 02:48Stimulation of Vascular Hyperpermeability
- 04:27Quantification of the Accumulated Evans Blue within the Dermis
- 06:22Results: Use of the Miles Assay to Detect Vascular Leakage Triggered by Intradermal Injection of VEGF into C57/BI6 Mice
- 07:39Conclusion
Here, we present a protocol to measure the vascular leakage induced by intradermal administration of permeability promoting agents into the murine skin. This technique can be used to determine the ability of molecules to promote or inhibit vascular leakage or to study the molecular mechanisms that regulate vascular permeability.
