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Homogeneous Time-resolved Förster Resonance Energy Transfer-based Assay for Detection of Insulin Secretion
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Homogeneous Time-resolved Förster Resonance Energy Transfer-based Assay for Detection of Insulin Secretion

Homogeneous Time-resolved Förster Resonance Energy Transfer-based Assay for Detection of Insulin Secretion

DOI:
10.3791/57531-v

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07:30 min

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May 10, 2018

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, ,
1Department of Psychiatry,University of Pittsburgh, 2Department of Cell Biology,University of Pittsburgh

Chapters

  • 00:05Title
  • 01:22INS-1E Cells: Maintenance and Plating
  • 03:04Insulin Secretion Assay
  • 04:12HTRF To Measure Insulin Secretion and Data Analysis
  • 05:17Results: Use of HTRF Assay for the Rapid Detection of Insulin Secretion from the Cells
  • 06:43Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present homogeneous time resolved FRET (HTRF) as an efficient method for rapid detection of insulin secreted from cells.

Tags

Keywords: Insulin SecretionTime-resolved FRETBeta CellsGlucose StimulationDiabetesDrug DiscoveryINS-1E CellsCell CultureTrypsinCell CountingViability

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