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Visualization of Amyloid β Deposits in the Human Brain with Matrix-assisted Laser Desorption/Ionization Imaging Mass Spectrometry
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Visualization of Amyloid β Deposits in the Human Brain with Matrix-assisted Laser Desorption/Ionization Imaging Mass Spectrometry

Visualization of Amyloid β Deposits in the Human Brain with Matrix-assisted Laser Desorption/Ionization Imaging Mass Spectrometry

DOI:
10.3791/57645-v

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09:31 min

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March 07, 2019

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, , , , , ,
1Department of Life and Medical Systems,Doshisha University, 2Bruker Daltonics K.K., 3The Brain Bank for Aging Research,Tokyo Metropolitan Geriatric Hospital and Institute of Gerontology, 4Graduate School of Brain Science,Doshisha University

Chapters

  • 00:04Title
  • 00:51Preparation of Tissue Sections for Imaging Mass Spectrometry (IMS)
  • 05:05MALDI-IMS
  • 06:12Results: Visualization of Aβ and Its Isoforms from Autopsied Brains with AD and CAA with MALDI-IMS
  • 08:14Conclusion

Summary

Automatic Translation

Automatic Translation

Molecular imaging with matrix-assisted laser desorption/ionization-based imaging mass spectrometry (MALDI-IMS) allows the simultaneous mapping of multiple analytes in biological samples. Here, we present a protocol for the detection and visualization of amyloid β protein on brain tissues of Alzheimer's disease and cerebral amyloid angiopathy samples using MALDI-IMS.

Tags

Keywords: Amyloid BetaMALDI Imaging Mass SpectrometryAlzheimer’s DiseaseAutopsyFormic AcidSegmentation MapsMarker ProteinsPeptidesPlaqueSubarachnoid VasculatureCryostatITO-coated Glass SlidesEthanol WashVacuum Drying

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