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JoVE Journal
Genetics

This content is Open Access.

Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines
 

Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines

Article doi: 10.3791/57883
June 2nd, 2018

Summary June 2nd, 2018

This protocol describes the steps needed to design and perform multiplexed targeting of enhancers with the deactivating fusion protein SID4X-dCas9-KRAB, also known as enhancer interference (Enhancer-i). This protocol enables the identification of enhancers that regulate gene expression and facilitates the dissection of relationships between enhancers regulating a common target gene.

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