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JoVE Journal
Biology

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Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9
 

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

Article DOI: 10.3791/52118
January 3rd, 2015

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Summary January 3rd, 2015

CRISPR/Cas9 is a robust system to produce disruption of genes and genetic elements. Here we describe a protocol for the efficient creation of genomic deletions in mammalian cell lines using CRISPR/Cas9.

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