Corneal Epithelial Abrasion with Ocular Burr As a Model for Cornea Wound Healing

This method can help answering key questions relating to the cellular and molecular mechanisms involved during the corneal wound healing. An advantage of this technique is that it reflects the situations in emergency room in case of corneal insert, meaning abrasion or scratch on the eye surface. I first had the idea for this method when I was studying corneal epithelial progenitor cells, and their behavior in a situation that challenges the homeostatic maintenance of the cornea.

Visual demonstration of this method is critical, because performing the corneal abrasion is difficult, and it requires practice. First, prepare a 0.1%Florecine solution for visualizing the abrasion under cobalt blue light by measuring 10 milligrams of Florecine salt with a fine scale. Then, add the salt to 10 milliliters of Fosfate buffered saline solution.