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An Epithelial Abrasion Model for Studying Corneal Wound Healing
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An Epithelial Abrasion Model for Studying Corneal Wound Healing

An Epithelial Abrasion Model for Studying Corneal Wound Healing

DOI:
10.3791/63112-v

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04:45 min

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December 29, 2021

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, , , ,
1College of Optometry,University of Houston, 2Children’s Nutrition Research Center,Baylor College of Medicine, 3Center for Translational Research on Inflammatory Diseases (CTRID),Michael E. DeBakey Veterans Affairs Medical Center

Chapters

  • 00:05Introduction
  • 00:47Preparation and Anesthesia
  • 01:34Creation of Corneal Wound, Monitoring of Wound Closure, and Re-Epithelialization
  • 03:30Results: Inflammatory Response and Wound Closure Following Central Corneal Epithelial Abrasion
  • 04:18Conclusion

Summary

Automatic Translation

Automatic Translation

Here, a protocol for creating a central corneal epithelial abrasion wound in the mouse using a trephine and a blunt golf club spud is described. This corneal wound healing model is highly reproducible and is now being used to evaluate compromised corneal wound healing in the context of diseases.

Tags

Keywords: Epithelial AbrasionCorneal Wound HealingCorneal InflammationCorneal Wounding ModelFluorescein StainingWound ClosureRe-epithelializationImage Analysis

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