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Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing
 

Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing

Article doi: 10.3791/58413
October 12th, 2018

Summary October 12th, 2018

Here, we present a protocol to rapidly isolate high-quality nuclei from the fresh or frozen tissue for downstream massively parallel RNA sequencing. We include detergent-mechanical and hypotonic-mechanical tissue disruption and cell lysis options, both of which can be used for isolation of nuclei.

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