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Direct Intrathecal Injection of Recombinant Adeno-associated Viruses in Adult Mice
 

Direct Intrathecal Injection of Recombinant Adeno-associated Viruses in Adult Mice

Article DOI: 10.3791/58565-v
February 15th, 2019

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Summary February 15th, 2019

Here we present a direct intrathecal injection technique using 1% lidocaine hydrochloride in a viral solution to ensure efficient adeno-associated virus delivery to small animals and establish a scoring system to predict transduction efficiency in the central nervous system according to the degree of transient weakness induced by lidocaine.

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