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Direct Intrathecal Injection of Recombinant Adeno-associated Viruses in Adult Mice
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JoVE Journal Neuroscience
Direct Intrathecal Injection of Recombinant Adeno-associated Viruses in Adult Mice

Direct Intrathecal Injection of Recombinant Adeno-associated Viruses in Adult Mice

DOI:
10.3791/58565-v

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08:17 min

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February 15, 2019

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, , , ,
1Department of Neurology,Second Hospital of Hebei Medical University, 2No.2 Middle School of Shijiazhuang, 3Department of Biochemistry and Molecular Pharmacology,University of Massachusetts Medical School, 4Key Laboratory of Hebei Neurology

Chapters

  • 00:04Title
  • 00:50Mouse Preparation and Direct Intrathecal Adeno-associated Virus (AAV) Delivery
  • 03:16Immunohistochemical Tissue Preparation
  • 04:46Immunohistochemistry
  • 06:27Results: Representative AAV Transduction After Direct Intrathecal Delivery
  • 07:52Conclusion

Summary

Automatic Translation

Automatic Translation

Here we present a direct intrathecal injection technique using 1% lidocaine hydrochloride in a viral solution to ensure efficient adeno-associated virus delivery to small animals and establish a scoring system to predict transduction efficiency in the central nervous system according to the degree of transient weakness induced by lidocaine.

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Keywords: Direct Intrathecal InjectionRecombinant Adeno-associated VirusesAdult MiceCentral Nervous System DiseasesALS27 Gauge NeedleHamilton SyringeGenome CopiesProne PositionBiosafety HoodPelvic GirdleL5-L6 Intervertebral SpaceMidline Of The Spine30-degree AngleIntradural Space

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