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Direct Intrathecal Injection of Recombinant Adeno-associated Viruses in Adult Mice
Direct Intrathecal Injection of Recombinant Adeno-associated Viruses in Adult Mice
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Direct Intrathecal Injection of Recombinant Adeno-associated Viruses in Adult Mice

Direct Intrathecal Injection of Recombinant Adeno-associated Viruses in Adult Mice

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08:17 min

February 15, 2019

DOI:

08:17 min
February 15, 2019

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Summary

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Here we present a direct intrathecal injection technique using 1% lidocaine hydrochloride in a viral solution to ensure efficient adeno-associated virus delivery to small animals and establish a scoring system to predict transduction efficiency in the central nervous system according to the degree of transient weakness induced by lidocaine.

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