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JoVE Journal
Neuroscience

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JoVE Journal Neuroscience
Direct Intrathecal Injection of Recombinant Adeno-associated Viruses in Adult Mice
 

Direct Intrathecal Injection of Recombinant Adeno-associated Viruses in Adult Mice

Article doi: 10.3791/58565
February 15th, 2019 •
*1, *2, 1, 3, 1,4
1Department of Neurology, Second Hospital of Hebei Medical University, 2No.2 Middle School of Shijiazhuang, 3Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, 4Key Laboratory of Hebei Neurology
* These authors contributed equally

Summary
Automatic Translation
February 15th, 2019

Here we present a direct intrathecal injection technique using 1% lidocaine hydrochloride in a viral solution to ensure efficient adeno-associated virus delivery to small animals and establish a scoring system to predict transduction efficiency in the central nervous system according to the degree of transient weakness induced by lidocaine.

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